ROS-Nitric Oxide Pathway Reagents and Kits
Revvity Health Sciences Inc Tubes with Screw Caps, 15 mL (500/pk)
Tubes with Screw Caps, 15 mL (500/pk)
Eagle Biosciences Inc Colorimetric Assay for Nitric Oxide Synthase
Colorimetric Nitric Oxide Assay Kit
Enzo Life Sciences L-NMMA . monoacetate (1g). CAS: 53308-83-1
NOS inhibitor. Competitively inhibits the generation of NO from arginine and is a useful tool for inhibition of NO mediated effects. In vitro, L-NMMA inhibits relaxation of rat aorta rings induced by acetylcholine (IC50=9.5µM). In vivo, it causes a dose dependent increase in mean arterial blood pressure in anesthetized rats (EC50=16.7mg/kg). Alternative name: NG-Monomethyl-L-arginine . monoacetate. Formula: C7H16N4O2 . CH3COOH. MW: 188.2 . 60.1. Purity: ≥99% (TLC). Appearance: White to off-white solid. Solubility: Soluble in water, 100% ethanol (1mg/ml) or DMSO (0.25mg/ml). Long Term Storage: +4°C.
Sigma Aldrich Fine Chemicals Biosciences Glyoxalase I Activity Assa
Glyoxalase I (GLO-1) a lactoylglutathione lyase also known as methylglyoxalase aldoketomutase ketone-aldehyde mutase and (R)-S-lactoylglutathione methylglyoxal-lyase is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria protozoans and cancer.
Abcam Nitric Oxide Assay Kit
Nitric Oxide Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical ROS DetectIon CelBased96
Cayman’s ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive control for ROS generation. N-acetyl-cysteine is included as an antioxidant control.
Enzo Life Sciences VASP monoclonal antibody (IE273) (50µg)
Clone: IE273. Isotype: IgG1. Immunogen: Purified human VASP (vasodilator stimulated phosphoprotein). Species reactivity: Human Rabbit, Porcine, Bovine. Specificity: Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of VASP. Applications: ICC, IP, WB. Formulation: Liquid. In PBS containing 2mg/ml BSA and 0.02% sodium azide.
Cayman Chemical AlnIn TrnsamInase ColorIme 96
Cayman’s Alanine Transaminase (ALT) Colorimetric Activity Assay Kit provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and cell lysates. Measurement of the ALT activity is carried out by monitoring the rate of NADH oxidation in a coupled reaction system employing lactate dehydrogenase (LDH). The oxidation of NADH to NAD+ is accompanied by a decrease in absorbance at 340 nm. Under circumstances in which the ALT activity is rate limiting, the rate decrease is directly proportional to the ALT activity in the sample.
Abcam DMNQ, non-alkyiating redo x cycling quinone, 25MG
MW 218.2 Da, Purity >99%. Cell-permeable, non-alkylating redox cycling quinone. Generates superoxide production and induces cell proliferation, apoptosis and necrosis in vitro. Redox cycling agent. Active in vivo and in vitro.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Patulin, fungal mycoto x in, 1MG
MW 154.12 Da, Purity >98%. Fungal mycotoxin, DNA damaging agent and induces ROS production. Apoptosis inducer via EGR-1 dependent ATF3 upregulation in vitro. Anti-bacterial, carcinogenic, mutagenic and anticancer agent. Active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences YC-1 (5mg). CAS: 170632-47-0
Nitric oxide (NO) independent, superoxide-sensitive stimulator of soluble guanylyl cyclase (sGC), EC50=18.6μM. Induces concentration-dependent increase in cGMP levels in cultured cells and in rabbit aortic rings. Inhibits platelet adhesion to collagen. Non-specific phosphodiesterase inhibitor. YC-1 inhibits HIF-α activity in tumors resulting in blocked angiogenesis and an inhibition of tumor growth. Inhibits Na+ channel.Binds to [3H]BW202W92. Purity: ≥98% (HPLC). Appearance: White to pale yellow powder. Solubility: Soluble in DMSO, methanol or dichloromethane. Long Term Storage: +4°C.
Cayman Chemical PhenylarsIn OxIde 250mg
An organoarsenic compound; inhibits insulin-induced PP1 activity and Glut4 translocation in rat adipocytes at 5 µM; completely inhibits insulin-induced PI3K activation and glucose transport in 3T3-L1 adipocytes at 30 and 50 µM, respectively; inhibits potassium chloride-induced exocytosis and induces mitochondrial depolarization in isolated rat brain synaptosomes; reduces LPS-induced neutrophil infiltration in BALF and carrageenan-induced paw edema in rats at 0.3 and 1 mg/kg, i.p
Abcam Cellular Membrane Potential Assay Kit (Fluorometric - Red).
Cellular Membrane Potential Assay Kit (Fluorometric - Red).
The product is subject to the following: Abcam Restricted Use Statement